Reverse Oxion OX.2253‑PLPHF

12.644,00€
VAT included

Inverted Fluorescence and Phase Contrast Microscope

45º inclined trinocular head
Pair of EWF10X/22mm eyepieces.
Diopter adjustment on the left eyepiece
Quintuple objective-carrying revolver
10x/0.30 Infinity Corrected Fluo Plan Objective (IOS)
Fluo plan objective with infinity correction (IOS) and 20x/0.45 and S40x/0.65 Phases.
XY mobile inverted stage (250x230 mm.) with coaxial controls in vertical position.
Includes accessories for Ø 35 mm cell culture dishes, standard sample holder and glass disc with central perforation.
Condenser for long working distances AN 0.30
Coaxial coarse and fine focusing knobs
3-position guide for 2 filter blocks
Epi-Fluorescence lighting system with HBO 100W mercury vapor lamp (high pressure) and orange UV screen
The following excitation filters are included:
- Blue excitation filter OX.9745, EX465-495 (BP) DM505 (LP) EM515-555 (BP)
- Green excitation filter OX.9746, EX540-580 (BP) DM600(LP) EM605-665 (BP)
Supplied with aluminium case

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SKU: OX.2253‑PLPHF
Description

Euromex Oxion Inverso inverted stage biological microscopes are available for brightfield, phase contrast and Epi-fluorescence applications. They are supplied with specific objectives that compensate for the 1.2 mm thickness of the bottom of Petri dishes or culture plates.

Oxion Inverso inverted microscopes are useful in life sciences for the observation of living organisms and tissue culture in both culture plates and culture flasks.

EYE CLOSURES
Pair of HWF10x/22mm wide field flat eyepieces.

HEAD
Trinocular with 45º inclined eyepiece tubes. Diopter adjustment on the left eyepiece holder. Adjustable interpupillary distance between 54 and 75 mm. Trinocular head with 0-100% light deviation.

REVOLVER LENS HOLDER
Quintuple objective-holder revolver in lateral folded position and mounted on high-precision ball bearings.

BRIGHT FIELD OBJECTIVES
(OX.2003-PL and OX.2053-PL models)

Long working distance infinity corrected plano-achromatic (IOS) objectives 10x/0.25, 20x/0.40 and 40x/0.60. Optional long working distance plano-achromatic (IOS) objectives 4x/0.10 and 60x/0.70 are available.

PHASE CONTRAST TARGETS

OX.9190r
(OX.2003-PLPH and OX.2053-PLPH models)

Long working distance infinity corrected (IOS) plano-achromatic and phase contrast objectives 10x/0.25, 20x/0.40, 40x/0.60.

A long working distance, 4x/0.13 phase contrast plano-achromatic (IOS) objective is optionally available.

(Photo: OX.9190 Foil with phase rings for 4x/10x objectives, phase rings for 20x/40x objectives and position for brightfield observation)

OBJECTIVES FOR BRIGHT FIELD AND EPI-FLUORESCENCE
(OX.2253-PLF and OX.2453-PLF models)

Long working distance infinity corrected (IOS) plan objectives 10x/0.30, 20x/0.45, 40x/0.65. Optionally available are a long working distance plan fluarex (IOS) objective 4x/0.13 and a 60x/0.75.

OBJECTIVES FOR PHASE CONTRAST AND EPI-FLUORESCENCE
( models OX.2253-PLPHF and OX.2453-PLPHF)

Long working distance 10x/0.30 infinity corrected (IOS) plan fluarex objective and long working distance 20x/0.45 and S40x/0.65 infinity corrected (IOS) plan fluarex phase contrast objectives.

All lenses are corrected to compensate for a thickness of 1.2 mm. All optical components have anti-mold treatment and anti-reflection coating treatment for maximum light transmission.

OX.9599rPLATINA (supplied on all models except OX.2003-PL and OX.2003-PLPH)
Large format stage 250 x 230 mm. with mobile X/Y carriage with 120 x 78 mm travel and vertical control knobs in right position, includes the following accessories:

  • Metal insert for cell culture dishes Ø 35 mm.
  • Support for standard 76 mm x 26 mm sample holders.
  • Glass disc with central perforation.
  • The universal support for culture plates (OX.9599) can be purchased optionally.

(Photo: OX.9599 Universal support for cell culture plates)

CONDENSER
AN 0.30 condenser with iris diaphragm. Working distance 72 mm. Phase contrast models OX.2003-PLPH, OX.2053-PLPH, OX.2253-PLPHF and OX.2453-PLPHF are supplied with the OX.9190 phase ring plate.

FOCUS CONTROLS
Coaxial macro and micrometer. 2 μm precision micrometer, total travel approximately 25 mm. With focusing tension adjustment.

LIGHTNING
Transmitted lighting using 5W NeoLed with adjustable intensity and internal 100-240 Vac power supply.

Epi-fluorescence models are equipped with a lighting system based on a 100W mercury vapor lamp and an external 100-240 Vac power supply with lamp life indication and an orange screen for UV protection.

LED LIGHTING FOR FLUORESCENCELE.6400
We have two LED sources for fluorescence with 4 or 9 excitation wavelengths.
Check out our products LE.6400-OX and LE.6900

FILTERS FOR PHASE CONTRAST MODELS
550 nm green interference filter and 45 mm diameter blue filter.

STANDARD EPI-FLUORESCENCE FILTER BLOCKS
(for Epi-fluorescence models)

The Epi-fluorescence models OX.2253-PLF and OX.2253-PLPHF are supplied with two excitation filters:

  • Blue excitation filter OX.9745, EX465-495 (BP) DM505 (LP) EM515-555 (BP)
  • Green excitation filter OX.9746, EX540-580 (BP) DM600(LP) EM605-665 (BP)

The Epi-fluorescence models OX.2453-PLF and OX.2453-PLPHF are supplied with a guide that allows a maximum of three excitation filters (in case you want to keep the bright field or the phase contrast) or a maximum of four excitation filters (if you only want to work in Epi-fluorescence), these models are supplied with three excitation filters:

  • Blue excitation filter OX.9745, EX465-495 (BP) DM505 (LP) EM515-555 (BP)
  • Green excitation filter OX.9746, EX540-580 (BP) DM600(LP) EM605-665 (BP)
  • Ultraviolet excitation filter OX.9747, EX361-389 (BP) D415 (LP) EM435-485 (BP)

SPECTRUM OF THE MOST COMMONLY USED FILTER BLOCKS

OX.9745_1

OX.9745 Blue excitation 465-495 nm
FITC EX465-495 (BP) DM505 (LP) EM515-555 (BP) 

Also suitable for: Acridine Orange + DNA, Alexa Fluor 488™, Atto 488, Azami Green, BODIPY FL/pH7.2, Calcein, Calcium Green™-1, Cy2™, DiO, DyLight 488, EGFP, Emerald GFP, FAM, FITC, Fluo-4, GFP LysoTracker Green/pH 5.2, MitoTracker Green FM/MeOH, mWasabi, Oregon Green™ 488, SYBR® Green I, SYTO 9/DNA, ZsGreen





OX.9746_1 OX.9746 Green excitation 540-580 nm
Texas Red / mCherry / Alexa Fluor 594 EX540-580 (BP) DM600 (LP) EM605-665 (BP)

Also suitable for: Alexa Fluor 568™, Alexa Fluor 594™, CAL Fluor® Red 610, Cy3.5™, DyLight 594, FusionRed, Killer Red, mCherry, MitoTracker Red/MeOH, mKate2, mRFP1, ReAsH-CCPGCC, Rhodamine Red ™-X, ROX, Sulforhodamine 101, Texas Red®, Texas Red®-X, X-rhod-1/Ca2+





OX.9747_1

OX.9747 UV excitation 361-381 nm
DAPI / Hoechst / Alexa Fluor 350 EX361-389 (BP) D415 (LP) EM435-485 (BP)

Also suitable for: Alexa Fluor 350™, Biosearch Blue, Coumarin, DAPI, DyLight 350, Hoechst 33258, LysoTracker Blue/MeOH






OX.9748_1

OX.9748 Violet excitation 361-389 nm
UV/DAPI Longpass, Calcofluor White EX415-445 (BP) DM455 (LP) EM465-495 (BP)









PACKAGING
Supplied with power cable, protective sheath, spare fuse, user manual. Models with 100W mercury vapor illumination include an orange screen for UV protection. Models with phase contrast include the telescopic eyepiece for centering. phases. Supplied with aluminium case

ICSI OR INTRACYTOPLASMIC SPERM INJECTION FOR REVERSE OXIDE

OX.2003-PLPH-ISCI

OX.2003-PLPH-ISCI_detail

(Photo: OX.2003-PLPH-ISCI Reverse Oxion with ICSI module for intracytoplasmic sperm injection

TECHNICAL SPECIFICATIONS

  • Perfect for users who need to practice this technique, as well as for professionals.
  • Fully manual system
  • Wide range of motion
  • Easy micropipette position at all times “Manual initial position”
  • Adjust the angle of the micropipette between 20º and 40º
  • Micropipette holder fixing knob reduces vibration and easy sperm handling
  • Easy installation and use
  • 3D hydraulic manipulation joysticks for smooth movement
  • Pneumatic air injector for ICSI and HOLDING
  • No injector service required
  • Sensitive injectors identical on both sides and interchangeable.
  • Great quality at a reasonable price
  • X, Y, Z movement range: 20mm each, and 1mm for a full turn of the controller
  • Optional, heating plate with temperature controller

(Photo: ISCI set (detail)

ISCI SET
The kit includes two 3D hydraulic joysticks, two pipette installation mechanisms, a connecting bar with a movable handle, two air injectors with tubing, a pipette holder, two metal plates for injectors, a set of Allen keys, a ruler for mechanical adjustment in the initial position. The unit is packed in a sturdy case.

HEATING PLATE

  • Heating glass plate for Euromex microscope stage with 110mm circular hole
  • The glass plate fits into the circular opening of the XY stage and allows a flat glass plate to be clamped at constant temperature.
  • Aluminum frame for greater strength and flatness
  • Glass thickness 0.5 mm. Plate diameter 110 mm

CONTROLLER

  • 2 channel temperature controller with touch screen interface
  • One controller can control up to two devices
  • The external temperature sensor (included) and self-calibration process allow easy calibration of heating devices
  • Data logging allows you to record temperature over time, save calibration and alarms.
  • The password protected controller is equipped with processes to calibrate the sensors against certified thermometers.

CHARACTERISTICS

  • Temperature range: from room temperature to 60 ° C
  • Temperature accuracy at sample: ±0.3°C
  • 4.3'' touch screen
  • External temperature sensor
  • Transferring the data log to a USB drive
  • Alarm sound and external alarm connector
  • One controller can control up to two devices
  • Self-calibration process allows for high temperature accuracy